To investigate the effect of temperature changes to the activity of Enzyme Catalyses.

Coursework: To investigate the centre of temperature changes to the practise of Enzyme Catalyses. By Marcus Final update: 26/11/03 Apparatus needed: Boiling tubes Beakers Bung with a deliverance tube Thermometer 5ml spray (To hive up potato) 10ml syringe (To mea certain the amount of solution) Diced potato Bunsen burner ( depose be replaced by thermosactic pee john if possible) Ice Glass rod Hydrogen bleach pH 7 original Variable and invariable factors: ØpH of enzyme: This can be postulate by the wreakition of pH7 buffer. Ø submergence of enzyme: This can be finished by using atomic number 1 peroxide from the alike line either time. ØThe time for reaction moldiness be the same all(prenominal) time ØSince the effect of changes in temperature to the enzyme activity is investigated in this try egress, all factors, still the temperature, has to be constant. I withdraw to repeat the experiment some(prenominal) times with a divers(prenominal) temperature of the substrate and the enzyme each time. The temperature elect be 10?,20?, 30?,40?,50?,60?. The figure away: 1.First or all, a turn tube is used to collect 5ml of diced potato with the 5ml syringe. 2.Add 10ml of pH7 buffer to the simmering tube to make sure pH is constant. 3.Pour 10ml of hydrogen peroxide into another stewing tube. 4.

Place both(prenominal) boiling tube in a pee can, add heat to the pee can until it reaches 80 ?. (For 10?. And 20?., which is probably tucker out beat than room temperature, ice is added to the water bath instead of heating.). Or If thermostatic water bath is available, the temperature can be changed by view the thermostatic water bath. When the temperature is reached, run through both boiling tubes out of the thermostatic water bath and continue the experiment. 5.Take both boiling tube out of the water bath, make sure that their temperature argon the same. 6.Get ready with... If you want to get a full essay, contrive it on our website: Ordercustompaper.com

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